Induction Of Banana Roots In Various Media And In Vitro Growth Regulators

Rina Srilestari, Suwardi Suwardi

Abstract


This research aims to determine the appropriate media and the best concentration of growth regulators. Furthermore, themethod used was a completely randomized design with two factors, namely the type of media (Murashige & Skoog, Media B5, and ½ MS + vitamin B5) and NAA (0.5; 1.0; 1.5 ppm) as well as BA (1; 2; 3 ppm. ). The data obtained were analyzed for diversity and further evaluation using Duncan Multiple Range Test (DMRT) at a 5% level. Furthermore, the result showed that Murashige & Skoog media interacts with NAA 1 ppm + BAP 2 ppm on root length parameters. Considering this result, media B5 is the best for plantlet roots number and dry weight parameters, while NAA 1.5 ppm and BA 3 ppm were the best plantlets dry weight concentrations.


Keywords


banana, media types, growth regulators, in vitro

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References


Abebe, Z., A. Mengesha, A. Teressa & W. Tefera. 2009. Efficient In Vitro Multiplication Protocol for Vanilla planifolia Using Nodal Explants in Ethiopia. African Journal of Biotechnology 8 (24): 6817-6821.

Anisa, N., S. Reine, W. Asnawati. 2016. Pengaruh BAP Terhadap Multiplikasi Tunas Anggrek Hitam (Coelogyne pandurata Lindl.) Secara Kultur Jaringan. Jurnal Hutan Lestari 4(4): 591-595

Bella, D. R. S., Suminar, E., Nuraini, A., & Ismail, A. 2016. Pengujian Efektivitas Berbagai Jenis dan Konsentrasi Sitokinin terhadap Multiplikasi Tunas Mikro Pisang (Musa paradisiaca L.) secara In Vitro. Jurnal Kultivasi, 15(2), 74-80.

BPS. 2019. Statistik Konsumsi Pangan Tahun 2018. Pusat Data dan Sistem Informasi Pertanian. Sekretariat Jendral Kementerian Pertanian.

Elma,T.A. E.Suminar., & S.Mubarok, A.Nuraini. .2017. Multiplikasi tunas mikro pisang (Musa paradisiaca L.) ‘Raja Bulu’ secara in vitro pada berbagai jenis dan konsentrasi sitokinin. Jurnal Kultivasi 16 (3) .

Ferdous, M.H., A.A.M. Billah, H. Mehraj, T. Taufique, & AFMJ Uddin. 2015. BAP and IBA pulsing for in vitro multiplication of Banana cultivars through shoot-tip culture. J.Bioscie. Agri. Research 3(2): 87-95.

Indria, W., Mansyur, & A. Husni. 2016. Pengaruh Pemberian Zat Pengatur Tumbuh 2,4- Dikhlorofenoksiasetat (2,4-D) Terhadap Induksi Kalus dan Penambahan Zat Pengatur Tumbuh Benzyl Adenine (BA) Terhadap Induksi Kalus Embriogenik Rumput Gajah Varietas Hawaii (Pennisetum purpureum Cv. Hawaii) (In Vitro). Jurnal Ilmu Ternak Universitas Padjadjaran 18(1): 34-46.

Kartiman, R., S. Dewi, I. A. Syarifah, dan P. Agus. 2018. Multiplikasi In Vitro Anggrek Hitam (Coelogyne pandurata Lindl.) pada Perlakuan Kombinasi NAA dan BAP. Jurnal Bioteknologi dan Biosains Indonesia 30(5): 75-87.

Kasutjianingati, Poerwanto R, Widodo, Khumaida N & Efendi D. 2017. Pengaruh Media Induksi terhadap Multiplikasi Tunas dan Pertumbuhan Planlet Pisang Raja Bulu (AAB) dan Pisang Tanduk (AAB) pada Berbagai Media Multiplikasi. J Agron Indonesia 39 (3) : 180-187

Mengesha, A., A. Biruk, G. Elias, dan T. Tewedros. 2012. Micro-Propagation of Vanilla planifolia Using Enset (Ensete ventricosum (Welw, Cheesman)) Starch as a Gelling Agent. Journal of Biological Sciences 4(4): 519-525.

Pranata, M. G., Y. Ahmad, dan P. Bambang. 2015. Pengaruh Konsentrasi NAA dan Air Kelapa Terhadap Multiplikasi Temulawak (Curcuma xanthorrizha Roxb.) Secara In Vitro. Journal of Sustainable Agriculture 30(2): 62-68.

Poerba YS, Imelda M dan Martanti D .2012. Analisis Kestabilan Genetik Pisang Kepok ‘Unti Sayang’ hasil mikropropagasi dengan marka RAPD dan ISSR. Berita Biologi. 11: 275-282. doi: 10.14203/berita biologi.v11i2.497

Widiastoety, D. 2014. Pengaruh Auksin dan Sitokinin Terhadap Pertumbuhan Planlet Anggrek Mokara (Effect of Auxin and Cytokinin on the Growth of Mokara Orchid Plantlets). J. Hort 24(3):230-238

UNCTS (Uganda National Council for Science and Technology). 2007. The Biology of Bananas and Plantains. Uganda National Council for Science and Technology (UNCTS) with Program for Biosafety Systems (PBS

Yelnititis. 2014. Perbanyakan Tunas Gyrinops versteegii (Gilg.) Domke. Jurnal Pemuliaan Tanaman Hutan 8(2): 108-120.




DOI: https://doi.org/10.31098/ess.v1i1.122

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